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Epifluorescent Fluorescence Single-Molecule Counting: Streptavidin-Labeled Phycoerythrin
Since the invention of the optical microscope, researchers have been striving to enhance the impact of target signals relative to background noise. Fluorophores have become mainstream signal-generating labels due to their brightness, well-defined excitation and emission profiles, reproducibility, and ease of use in biological systems. However, the use of fluorophores also introduces certain limitations.
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2025 10 / 20 |