| Detailed description |
| Product Name |
Wheat germ agglutinin, a lectin derived from common wheat (wheat)，Wheat Germ Agglutinin ，WGA |
| Product Introduction |
The receptor sugar for WGA is N-acetylglucosamine, and it preferentially binds dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, a structure shared by many serum and membrane glycoproteins. Bacterial cell wall peptidoglycan, chitin, cartilage glycosaminoglycans and glycolipids can also bind WGA. It has been reported that native WGA also interacts with some glycoproteins via sialic acid residues. |
| Specifications |
1mg/5mg/25mg/100mg/500mg/1g |
| Molecular weight |
36Kd |
| Extinction coefficient |
1.46 |
| Form |
Lyophilized powder |
| Usage Method |
It is recommended to dissolve it using phosphate buffer at pH 6.8 or HEPES buffer at pH 8.5. For long-term storage, 0.08% sodium azide may be added. |
| Storage conditions |
Store at -20℃. After thawing, keep at 2-8℃. Do not re-freeze. |
| Carbohydrate binding specificity |
Oligosaccharides of dimeric or trimeric N-acetylglucosamine or chitobiose |
| Activity |
The minimum concentration that can agglutinate a 2% rabbit red blood cell suspension incubated at 25°C for 1 hour < 20 μg/ml |
| Purity |
＞95%（SDS-PAGE）
|
| Source |
Common wheat |