| Detailed description |
| Product Name |
Canavalia ensiformis agglutinin，Concanavalin A，ConA |
| Product Introduction |
Con A is a plant lectin purified from jack bean. It is a tetrameric protein consisting of four identical subunits. Con A can bind to mannose residues of various glycoproteins and stimulate lymphocytes. Concanavalin A has no blood group specificity, but it shows affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Its activity requires the presence of Ca2+ and Mn2+ ions. Concanavalin A dissociates into dimers at pH 5.6 or below. It exists as a tetramer at pH between 5.8 and 7.0, and forms higher polymers when the pH is above 7.0. Depending on the degree of polymerization, concanavalin A may exhibit mitogenic activity. Succinylation can produce a stable and active dimer form. |
| Specifications |
1mg/5mg/25mg/100mg/500mg/1g |
| Molecular weight |
104 kD |
| Extinction coefficient |
1.2 |
| Form |
Lyophilized powder |
| Usage Method |
It is recommended to dissolve in a buffer containing 10 mM HEPES and 0.1 mM CaCl2 at pH 8.5. For long-term storage, 0.08% sodium azide may be added. |
| Storage conditions |
Store at -20℃, keep at 2–8℃ after thawing. Do not refreeze. |
| Carbohydrate binding specificity |
It has affinity for terminal α-D-mannosyl and α-D-glucosyl residues |
| Activity |
The minimum concentration at which a 2% rabbit red blood cell suspension incubated at 25°C for 1 hour can agglutinate is < 20 μg/ml |
| Purity |
＞90%（SDS-PAGE)
|
| Source |
jack bean |